C2C ELISA Collagen Type II Cleavage Assay

Intended use:

The C2C ELISA kit measures cartilage degradation. This assay with specific monoclonal antibody to type II collagen primary cleavage site recognizes the α-chain fragments containing an approximate 9 amino acid sequence from the carboxy terminus of the three-quarter length piece produced by collagenase cleavage (MMP-1, MMP-8, MMP-13) of type II collagen.

Click here – to download the C2C Specification Sheet

Method:                      ELISA competitive inhibition, 96 wells

Sample type:              Optimized for serum (may also be used for plasma, synovial fluid, urine, cell culture, cartilage extracts)

Sample volume:        25 µL per replicate

Calibration range:     10 – 1000 ng/mL

Incubation time:        2.5 hours

Species:                     Optimized for human (may also be used for various animal species)

Cross-reaction:         No cross-reaction with uncleaved triple-helical and heat-denatured human collagen types I and II and uncleaved alpha-chains of collagen types I and II. No cross-reaction with correspondingly cleaved alpha-chains of collagen type I